The papaya is the fruit of the plant Carica papaya, the sole species in the genus Carica of the plant family Caricaceae. The papaya is a large, tree-like plant, with a single stem growing from 5 to 10 m (16 to 33 ft) tall, with spirally arranged leaves confined to the top of the trunk. The lower trunk is conspicuously scarred where leaves and fruit were borne.It consists of high content of zeaxthin, proteolytic enzymes like papain and chymo papain, vitmin K, β-carotene, other phytoconstituents like polyphenols, sugars, aromatic amino acids and sulphur containing amino acids, phytosterols, starch and nutrients e. g P, S, K, Ca, Fe, Mg etc and had good health protective effects. The main objective of the present work is to evaluate the anticancer activity of various extracts of fruit of Carica papaya against Human Prostate cancer cell line DU-145. DU-145 and PC3 human prostate cancer cell lines are the “classical” cell lines of prostatic cancer DU145 cells have moderate metastatic potential compared to PC3 cells which have high metastatic potential The DU145 cell line was derived from brain metastasis. SRB assay was used to analyze the cell growth inhibition. From the present studied it had been displayed that CFE, ELE and MLE, all were exhibiting the potential capability to kill the cancer cell when compared with standard drug 5-FU. The cell growth inhibition by various extracts of fruit of Carica papaya was varied due to the presence of varying concentration of bioactive compounds. CFE had displayed the highest cell growth inhibition (94.52 %) at 6.25 µg (IC50 = 2.2 µg/ml) due to the presence of polyphenols (flavanoids), ELE with the 93.83% growth inhibition at 6.25 µg (IC50 = 2.4 µg/ml) and MLE with the 92.80% % growth inhibition at 6.25 µg (IC50 = 2.6 µg/ml).