Chrysanthemum plant is an ayurvedic important medicinal plant, and modern systems of medicine, flowers are the most important part in the field for preparation of various drugs. The present study aimed to focused objective was to study the two flower extracts with methanol and ethanol solvents and its antimicrobial activity both bacteria (Staphylococcus and Pseudomonas strains) and fungi (Candida species). When the C. indicum plant leaf extract treated with the experimental organisms of three strains Pseudomonas strains clearly showed that the maximum and minimum zone of inhibition was noticed on PA-38, 8±1.38 and PA- 37, 4±0.11. Similarly, ethanolic extract illustrated that the higher activity of this strain PA-39, 11±2.68 as well as PA-38, revealed the observed the value of 9±0.65 minimum effect on ethanolic extract. While, the C. cinerariaefolium ethanolic flower extract was treated with three different strains of Pseudomonas maximum similar zone of inhibition was noticed against PA-37 (10 ±2.75) and PA-38 (10 ±2.65). It was statistically significant as well as highest response when compared with Ciprofloxacin. Amoung the two plants C. indicum possessed excellent antimicrobial activity on both bacteria and fungi than the C. cinerariaefolium plant leaf. Subsequently the present research analyzed flower of the C. indicum plant by GC-MS techniques in order to determine the majority compounds. Totally 18 compounds were analyzed, amoung the 18 analytes camphor is an important peak compound also it contains 19.5 and 0.60% retention time and abundance respectively. Furthermore C. indicum possessed second most compound is Isoborneol, it was probably noticed 0.410% of abundance and its retention time is 17.5. Additionally two compounds also been observed as a sub peak level such as γ- Cadinene Methyl ester and Chrysanthenol. Hence, the current result clearly showed that the C. indicum plant flower is act as a very good potential antimicrobial agent.