Urinary Tract Infection (UTI) remains the most common bacterial infection in human population. The squealae of complicated UTIs are substantial. It is well established that in the presence of obstruction, calculus, Diabetes mellitus, and other risk factors, UTIs can lead to progressive renal damage. The aim of this study was to determine bacterial agents responsible for urinary tract infection and to evaluate their susceptibility pattern. Urine sample was cultured on Cystine Lactose Electrolyte Deficient (CLED) media by calibrated loop technique. Antimicrobial susceptibility testing was performed by Kirby bauer disc diffusion method. Out of 320 samples, 155 samples revealed growth of one type of bacteria, and 15 showed growth of 2 types of bacteria. E. Coli was most common bacteria (115/185=62.16%) followed by other enterobacteriaceae, Pseudomonas and a few gram positive cocci. Many bacteria were found to be multidrug resistant while most of gram negative bacteria were susceptible to Imipenem, and most of gram positive bacteria were susceptible to linezolid.